Identification of phytochemical constituents from biologically active pet ether and chloroform extracts of the flowers of Allamanda violacea A.DC (Apocynaceae)

Allamanda violacea is an ornamental plant of the genus Allamanda belongs to family Apocynaceae. Different flower extracts of the plant have been reported to possess anti-hyperlipidemic, anti-hyperglycemic and anti-oxidant activities. Present study deals with the identification of phytochemical constituents of the petroleum ether (pet ether) and chloroform extract by GC-MS analysis, and the constituents responsible for difference in the biological activity of these extract. Number of underivatized constituents identified in the pet ether and chloroform extracts, by comparing with the reference spectra’s of NIST library, were found to be 11 and 10 respectively. The respective extracts on derivation with N, Obis (trimethylsilyl) trifluoroacetamide (BSTFA) + trimethylchlorosilane (TMCS) [99:1 v/v] yielded their trimethyl silane derivatives. Sixteen constituents from the pet ether fraction and fourteen from chloroform fractions were identified after derivation.